HPA018297

Anti-USP25 antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• 別名: Anti-Deubiquitinating enzyme 25, Anti-USP on chromosome 21, Anti-Ubiquitin carboxyl-terminal hydrolase 25, Anti-Ubiquitin thioesterase 25, Anti-Ubiquitin-specific-processing protease 25
• UNSPSCコード: 12352203
• Human Protein Atlas Number: HPA018297
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 製品種目: Prestige Antibodies^® Powered by Atlas Antibodies
• 形状: buffered aqueous glycerol solution
• 化学種の反応性: human
• 強化検証: RNAi knockdown; Learn more about Antibody Enhanced Validation
• テクニック: immunoblotting: 0.04-0.4 μg/mL; immunohistochemistry: 1:200-1:500
• 免疫原配列: QPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSIMMTPNMQGIIMAIGKSRSVYDRCGPEAGFFK
• UniProtアクセッション番号: Q9UHP3
• 輸送温度: wet ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... USP25(29761)

詳細

詳細

The gene USP25 (ubiquitin-specific-processing protease 25) has been mapped to human chromosome 21q11.2. It contains three ubiquitin binding domains at the amino-terminus: one ubiquitin-associated domain and two ubiquitin-interacting motifs. USP25 encodes three different protein isoforms produced by alternate splicing: two are expressed ubiquitously and the longest is restricted to muscle tissues. USP25 is mainly present in the cytoplasm.

免疫原

ユビキチンC末端ヒドロラーゼ25のPrEST(protein epitope signature tag)抗原リコンビナントタンパク質。

アプリケーション

Prestige抗体^®は、Human Proteome Resource(HPR)プロジェクト(www.proteinatlas.org)によって開発・実証されたAtlas抗体です。抗体はすべて、数百の正常組織・疾病組織に対する免疫組織染色試験を行っています。これらの染色画像はHuman Protein Atlas(HPA)サイトで[Image Gallery]リンクをクリックするとご覧いただけます。さらに、ほとんどのPrestige抗体はプロテインアレイおよびウェスタンブロッティングの試験を行っています。試験のプロトコールおよびPrestige抗体、HPAに関する情報はsigma.com/prestigeをご覧ください。

生物化学的／生理学的作用

Ubiquitin-specific-processing protease 25 (USP25) is a specific-ubiquitin protease gene. SUMOylation of USP25 impairs its activity by reducing the affinity to ubiquitin chains. In muscle tissues, USP25 is up-regulated during myogenesis. USP25 interacts with myosisn binding protein C1 and protects the latter from degradation which is important in muscle differentiation and maintenance. USP25 is a negative regulator of the virus-triggered type I interferon (IFN) signalling pathway. RT-PCR analysis showed up-regulation of USP25 in Down′s syndrome fetal brains in mouse model. USP25 is down-regulated in human lung cancer. MiR-200c exerts tumor-suppressive effects of non-small-cell lung cancer though suppression of USP25 expression.

特徴および利点

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

関連事項

Corresponding Antigen APREST73924

物理的形状

PBS溶液(pH 7.2, 40%グリセロールおよび0.02%アジ化ナトリウム含有)。

法的情報

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)