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包装
pkg of 1 kit
メーカー／製品名
Cytiva RPN5785
色
ECF substrate: Off-white/white to yellowish ECF substrate dilution buffer: Colorless
輸送温度
wet ice
保管温度
2-8°C
詳細
Alkaline phosphatase dephosphorylates ECF™ Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.
The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.
The following reagents are provided, sufficient for detecting 2500 cm2 membrane: ECF™ Substrate, 60 mL, and dilution buffer.
アプリケーション
Vistra ECF™ substrate has been used in Western blotting.
特徴および利点
- A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments (see Storm)
- For use in western blotting, dot, and slot blotting applications
- Sensitivity comparable to chemiluminescence
- The wider dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
- Eliminates need for darkroom and film
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
ECF is a trademark of Cytiva
シグナルワード
Danger
危険有害性情報
保管分類コード
12 - Non Combustible Liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Anti-atherosclerotic effects of small molecular weight compounds enhancing eNOS expression and preventing eNOS uncoupling.
Wohlfart P, et al.
Journal of Pharmacology and Experimental Therapeutics (2007)
Cindy Gueguen et al.
PloS one, 9(2), e87367-e87367 (2014-03-04)
Poly(ADP-ribose)polymerase and sirtuin 1 are both NAD(+)-dependent enzymes. In vitro oxidative stress activates poly(ADP-ribose)polymerase, decreases NAD(+) level, sirtuin 1 activity and finally leads to cell death. Poly(ADP-ribose)polymerase hyperactivation contributes to cell death. In addition, poly(ADP-ribose)polymerase inhibition restores NAD(+) level and
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