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Key Documents

GERPN2232

ECL Prime Western Blotting System

Cytiva RPN2232

別名:

Amersham ECL Prime, Chemiluminescent Western blotting kit, chemiluminescent detection reagent

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About This Item

UNSPSCコード:
41105332
NACRES:
NA.32

包装

pkg of 1 kit

メーカー/製品名

Cytiva RPN2232

Detection reagent 1: Colorless Detection reagent 2: Colorless

関連するカテゴリー

詳細

Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.

Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.

アプリケーション

Sensitive quantitative or confirmatory Western Blotting

特徴および利点

  • High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
  • Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
  • Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm.
  • Compatible with Rainbow Molecular Weight Markers and Amersham ECL DualVue Western Blotting Markers.
  • Optimized for use with Amersham Hybond-P (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
  • Recommended membrane Hybond-P or Hybon ECL

構成

The following components are included in the Amersham ECL Prime detection reagent kit.
  • RPN2232 Solution A: Luminol solution, 50 ml Solution B: Peroxide solution, 50 ml sufficient for 1000 cm2 membrane

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

Color:
Detection reagent 1: colorless
Detection reagent 2: colorless

Sensitivity: ~1 pg

法的情報

ECL Prime
This product or portions thereof is manufactured and sold under license from Cyanagen Srl and is subject of US patent 7,855,287, US Patent 7,803,573, and Italian application number TO2010A000580, together with other equivalent granted patents and patent applications in other countries.
Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Rainbow is a trademark of Cytiva

関連製品

製品番号
詳細
価格

保管分類コード

10 - Combustible liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Yana van der Weegen et al.
Nature communications, 11(1), 2104-2104 (2020-05-02)
The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA
Cody J Warren et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(23), 11460-11469 (2019-05-23)
Pandemic HIV-1 (group M) emerged following the cross-species transmission of a simian immunodeficiency virus from chimpanzees (SIVcpz) to humans. Primate lentiviruses (HIV/SIV) require the T cell receptor CD4 to enter into target cells. By surveying the sequence and function of

資料

Troubleshooting of Detection Methods of GST-tagged Proteins

プロトコル

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

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