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資料

GE80-6483-56

2-D Quant Kit

Cytiva 80-6483-56

別名:

2D Quantification Kit, Protein Quant Kit

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About This Item

UNSPSCコード:
41105323
NACRES:
NA.25

特徴

wetted part: no

メーカー／製品名

Cytiva 80-6483-56

包装

pkg of 500 assays

保管温度

2-8°C

関連するカテゴリー

詳細

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

Common spectrophotometric methods of quantitating protein rely either on Coomassie dye binding or protein-catalyzed reduction of cupric (Cu2+) ion to cuprous (Cu+) ion. Dye-binding assays cannot be used in the presence of any reagent that also binds the dye. This includes carrier ampholytes and detergents such as PlusOne CHAPS, SDS, and Triton X-100. Assays that depend on the reduction of cupric ion cannot be used in the presence of reductants such as DTT, or in the presence of reagents that form complexes with cupric ion, such as thiourea or EDTA.

The 2-D Quant Kit procedure works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein. Precipitated proteins are resuspended in a copper-containing solution and unbound copper is measured with a colorimetric agent. The absorbance at 480 nm is inversely related to the protein concentration. The assay has a linear response to protein in the range of 0 to 50 μg and recommended sample volume is 1 to 50 μ.

特徴および利点

  • Accurately determine protein concentration in the presence of 2% SDS, 1% DTT, 8 M urea, 2 M thiourea, 4% CHAPS, 2% Pharmalyte, and 2% IPG Buffer.
  • Quantitatively precipitates proteins while leaving interfering substances behind.
  • Linear response in the range of 0 to 50 μg protein, with recommended sample volumes of 1 to 50 μ.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

ピクトグラム

Environment
GHS09

シグナルワード

Danger

危険有害性情報

H314,H335,H411

保管分類コード

8B - Non-combustible corrosive hazardous materials

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Milan O Urban et al.
Frontiers in plant science, 12, 628167-628167 (2021-06-29)
The present study aims to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at the proteome level. The PEG-induced osmotic stress was studied in the cotyledonary stage of MDE of two genotypes: Cadeli (D) and Viking (V)

プロトコル

Protein Determination using 2-D Quant Kit

2-D Quant Kit from GE Healthcare Lifescience is designed to accurately determine protein concentrations in samples for electrophoresis.

Protein Determination using 2-D Quant Kit

2-D Quant Kit from GE Healthcare Lifescience is designed to accurately determine protein concentrations in samples for electrophoresis.

Desalting Samples Using Mini Dialysis Kit

Mini Dialysis Kit from GE Healthcare Lifescience is designed for the dialysis of small sample volumes with minimal handling and sample loss and reduces the pronounced streaking on 2-D gels caused by low-molecular-weight contaminants.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from GE Healthcare is designed to prepare samples that would otherwise produce poor 2-D results.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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