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ligand
dextrin
包装
pack of 1 mL
メーカー/製品名
Cytiva 29-0486-41
保管条件
(20% Ehtanol)
パラメーター
42 psi
ベッドサイズ
7 mm × 25 mm
ベッド容積
1 mL
カラム内径
7 mm
サイズ
平均部品サイズ
34 μm
cleaning
2-13
working range
>7
キャパシティ
~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
保管温度
2-8°C
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詳細
MBPTrap™ HP columns prepacked with Dextrin Sepharose™ High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP). This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
特徴および利点
- HiTrap™ columns prepacked with Dextrin Sepharose™ High Performance medium.
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
- Physiological conditions and mild elution preserve the activity of the target protein.
- Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
その他情報
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法的情報
MBPTrap is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows troubleshooting of methods for purification of MBP-tagged recombinant proteins using GE Healthcare products.
This page shows the characteristics of Dextrin Sepharose High Performance products from GE Healthcare.
プロトコル
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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