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Merck

GE28-9909-46

Sigma-Aldrich

Superdex® 200 Increase 3.2/300

column L × I.D. 30 cm × 3.2 mm, 8.6 μm particle size

別名:

Superdex 200 Increase

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About This Item

UNSPSCコード:
41115700
NACRES:
SB.52

物質

glass column
titanium frit

製品種目

Superdex®

分子量

10000-600000 Da (globular proteins)

包装

1 ea of

パラメーター

<0.15 ml/min flow rate
3.0 Mpa (652 psi)
4-50 mL sample volume

ベッド容積

~2.4 mL

カラムL × 内径

30 cm × 3.2 mm

マトリックス

agarose-dextran composite
polymeric

粒径

8.6 μm

cleaning

1-14

working range

3-12

分離法

size exclusion (SEC)

詳細

Superdex 200 is a column for high resolution gel filtration in micro scale (μg) preparative purification as well as characterization and analysis of small sample volumes (4-50 μL) of proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

文献

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification as well as characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

適合性

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
for both preparative and analytical purposes

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

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法的情報

Superdex is a registered trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

This page shows how to perform a purification of His-tagged membrane proteins.

プロトコル

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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