物質
glass column
glass fiber (polypropylene reinforced)
分子量
3000-70000 Da (globular proteins)
包装
pkg of 1 ea
メーカー/製品名
Cytiva 28-9893-34
パラメーター
<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa (42 psi)
ベッド容積
320 mL
カラムL × 内径
60 cm × 26 mm
マトリックス
agarose dextran composite
dextran-agarose particle platform
polymeric
マトリックス活性基
dextran-agarose phase
dextran-agarose phase
平均部品サイズ
34 μm
cleaning
1-14
working range
3-12
分離法
size exclusion (SEC)
詳細
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
特徴および利点
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
適合性
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
プロトコル
This page shows how to refold proteins using gel filtration and products from GE Healthcare.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら(テクニカルサービス)