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Merck

GE28-9852-54

rProtein A GraviTrap

Cytiva 28-9852-54, pack of 10 × 1 mL

別名:

GraviTrap

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

形状

prepacked column

クロスリンク

cross-linked

包装

pack of 10 × 1 mL

メーカー/製品名

Cytiva 28-9852-54

保管条件

(20% Ethanol)

テクニック

liquid chromatography (LC): suitable

ベッド容積

1 mL

マトリックス

(Highly cross-linked agarose, 4%)

マトリックス活性基

Protein A functional group

粒径

(90 µm)

キャパシティ

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

分離法

affinity

保管温度

2-8°C

詳細

rProtein A GraviTrap includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose Fast Flow.

アプリケーション

rProtein A GraviTrap are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

特徴および利点

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as rProtein A GraviTrap ~ 50 mg human IgG/column

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

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資料

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

すべて表示

プロトコル

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

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