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Merck

GE28-9523-60

GST GraviTrap

Cytiva 28-9523-60

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About This Item

UNSPSCコード:
41106501
NACRES:
NA.56

ligand

glutathione

包装

pkg of 10 ea

メーカー/製品名

Cytiva 28-9523-60

ベッド容積

2 mL

カラム内径

15 mm

Matrix

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

4-13

動作範囲

4-13

キャパシティ

≥20 mg binding capacity(recombinant glutathione S-transferase (rGST))
>25 mg binding capacity (horse liver GST/ml medium)

詳細

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST GraviTrap contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein.

アプリケーション

GST GraviTrap contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

特徴および利点

  • Convenient, prepacked gravity-fLow columns.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • Yields up to 20 mg of pure Proteins per column.
  • Yields up to 20 mg of pure Proteins per column

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

プロトコル

Characteristics of Glutathione Sepharose® Products

Characteristics of Glutathione Sepharose® Products

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