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ligand
quaternary amine
詳細
Ion Exchanger Type (value)
包装
pkg of 1 ea
メーカー/製品名
Cytiva 28-9505-10
パラメーター
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
ベッドサイズ
7.7 mm × 100 mm
ベッド容積
4.7 mL
カラム内径
7.7 mm
マトリックス
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14
working range
2-12
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
HiScreen™ Q FF columns are prepacked with Q Sepharose™ Fast Flow, a strong anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.
アプリケーション
The small column volume of 4.7 mL and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.
特徴および利点
- Q Sepharose™ Fast FLow is a strong anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
プロトコル
This page discusses how to do a purification with SOURCE™ media from GE Healthcare
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