おすすめの製品
包装
pkg of 1 ea
メーカー/製品名
Cytiva 28-9365-51
パラメーター
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
ベッドサイズ
16 mm × 100 mm
ベッド容積
20 mL
カラム内径
16 mm
マトリックス
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
キャパシティ
~40 mg binding capacity(histidine-tagged protein)
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
特徴および利点
- Optimized for convenient scale-up purification of histidine-tagged proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible leakage of Ni2+.
- High binding capacity, approx. 40 mg/mL medium.
- Convenient and time-saving 20 mL prepacked HiPrep™ format.
保管および安定性
アナリシスノート
その他情報
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
プロトコル
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
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