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Key Documents

GE28-9365-51

Hisprep Fast Flow 16/10

Cytiva 28-9365-51

別名:

16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column

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About This Item

UNSPSCコード:
23151817
NACRES:
NA.56

包装

pkg of 1 ea

メーカー/製品名

Cytiva 28-9365-51

パラメーター

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

ベッドサイズ

16 mm × 100 mm

ベッド容積

20 mL

カラム内径

16 mm

マトリックス

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

キャパシティ

~40 mg binding capacity(histidine-tagged protein)

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

HisPrep FF 16/10 columns are prepacked with Ni Sepharose 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

特徴および利点

  • Optimized for convenient scale-up purification of histidine-tagged proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible leakage of Ni2+.
  • High binding capacity, approx. 40 mg/mL medium.
  • Convenient and time-saving 20 mL prepacked HiPrep format.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

HiPrep is a trademark of Cytiva
HisPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Environment

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

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資料

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

プロトコル

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

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