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GE28-9365-50

GSTPrep™ Fast Flow 16/10

Cytiva 28-9365-50

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About This Item

UNSPSCコード:
13111000
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

包装

pkg of 20 mL

メーカー／製品名

Cytiva 28-9365-50

パラメーター

<10 mL/min flow rate
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

ベッドサイズ

16 mm × 100 mm

ベッド容積

20 mL

カラム内径

16 mm

Matrix

highly cross-linked 4% agarose

平均直径

90 μm

cleaning

3-12

動作範囲

3-12

キャパシティ

>10 mg/mL binding capacity

詳細

GSTPrep™ FF 16/10 is prepacked, ready-to-use column containing Glutathione Sepharose™ 4 Fast FLow designed for one-step, scale-up purification of glutathione S-transferase (GST)-tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

アプリケーション

Glutathione Sepharose™ 4 Fast FLow is for scale-up purification of recombinant GST-tagged Proteins, other glutathione S-transferases, and glutathione-dependent Proteins. Mild elution conditions preserve Protein antigenicity and function.

特徴および利点

  • Fast and easy one-step, scale-up purification of GST-tagged Proteins.
  • Binds approx. 200 mg recombinant GST/column
  • Mild elution conditions preserving Protein antigenicity and function.
  • Simple operation with a pump or chromatography system, such as ÄKTA® design.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

GSTPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

ピクトグラム

Flame
GHS02

シグナルワード

Warning

危険有害性情報

H226

保管分類コード

3 - Flammable liquids

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試験成績書（COA）

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 資料 section.

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資料

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

すべて表示

プロトコル

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Performing a Separation with GE Healthcare Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B columns

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B columns

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）