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物質
polycarbonate housing
polyethersulfone membrane
無菌性
non-sterile
シェルフライフ
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包装
pack of 25 pieces
メーカー／製品名
Cytiva 28-9322-58
利用可能性
not available in Japan
チューブサイズ
~15 mL
有効容積
2 mL
ポアサイズ
100 kDa MWCO
保管温度
room temp
詳細
Designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%.
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest
特徴および利点
- Compatible pH range is from pH 1 to 9.
- Patented dead-stop technology, which ensures that samples cannot be concentrated to dryness and enables direct concentrate recovery
- One-step sample concentration in a single tube for minimal sample handling and reduced sample loss.
- Vertical polyethersulfone membrane minimizes membrane blockage and tolerates high flow rates.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Vivaspin is a registered trademark of Sartorius Stedim Biotech GmbH
保管分類コード
13 - Non Combustible Solids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Hyesook Yoon et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(7), 1483-1500 (2020-01-09)
Myelin loss limits neurological recovery and myelin regeneration and is critical for restoration of function. We recently discovered that global knock-out of the thrombin receptor, also known as Protease Activated Receptor 1 (PAR1), accelerates myelin development. Here we demonstrate that
資料
This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.
プロトコル
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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