GE28-4083-47

Ab Spintrap^™

Cytiva 28-4083-47

• UNSPSCコード: 41106500
• NACRES: NA.25

特性

• ligand: recombinant protein G lacking albumin-binding region
• 包装: pkg of 50 × 100 μL
• メーカー／製品名: Cytiva 28-4083-47
• 保管条件: (20% Ethanol)
• ベッド容積: 100 μL
• Matrix: highly cross-linked 6% agarose
• 平均部品サイズ: 34 μm
• cleaning: 2-9
• 動作範囲: 3-9
• キャパシティ: >1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.); >1 mg binding capacity(IgG/column)
• 保管温度: 2-8°C

詳細

詳細

Ab SpinTrap^™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

アプリケーション

Ab SpinTrap^™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap^™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap^™ contains Protein G Sepharose^™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap^™ together with buffers prepared using Ab Buffer Kit.

特徴および利点

• Rapid and convenient small-scale purification of IgG.
• Protein G Sepharose^™ High Performance in prepacked spin columns.
• No filtration or centrifugation of different crude samples necessary for antibody purification.
• Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
• Elution conditions formatted for both LC-MS and electrophoresis analysis.
• Elution conditions formatted for both LC-MS and electrophoresis analysis.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids