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Key Documents

GE28-4017-45

GSTrap^™ 4B

Name: GSTrap<SUP>™</SUP> 4B
Brand: SIGMA

Cytiva 28-4017-45, pack of 5 × 1 mL

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About This Item

UNSPSCコード:

23151816

NACRES:

NA.56

ligand

glutathione

形状

prepacked column

クロスリンク

cross-linked

包装

pack of 5 × 1 mL

メーカー／製品名

Cytiva 28-4017-45

保管条件

(20% Ethanol)

パラメーター

<4 mL/min flow rate
42 psi (H₂O at 25°C)

テクニック

liquid chromatography (LC): suitable

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

マトリックス

4% agarose

マトリックス活性基

glutathione functional group

粒径

45-165 μm

平均直径

90 μm

cleaning

4-13

working range

4-13

キャパシティ

>25 mg binding capacity (horse liver GST/ml medium)

分離法

affinity

詳細

GSTrap^™ 4B columns are prepacked Glutathione Sepharose^™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

アプリケーション

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA^® design or FPLC^™ System.

特徴および利点

High binding capacity.
Mild elution conditions preserving Protein antigenicity and function.
Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
GSTrap^™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA^® design.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.