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Key Documents

GE17-5282-02

GSTrap High Performance

Cytiva 17-5282-02, pack of 5 × 5 mL

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About This Item

UNSPSCコード:
23151817
NACRES:
NA.56

ligand

glutathione

包装

pack of 5 × 5 mL

メーカー/製品名

Cytiva 17-5282-02

パラメーター

<20 mL/min flow rate
42 psi (H2O at room temperature.)

ベッドサイズ

16 mm × 25 mm

ベッド容積

5 mL

カラム内径

16 mm

マトリックス

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

3-12

working range

3-12

キャパシティ

>7 mg/mL binding capacity (medium)

関連するカテゴリー

詳細

GSTrap HP columns are prepacked with Glutathione Sepharose High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

GSTrap HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

特徴および利点

  • High resolution purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Fast, one-step purification.
  • Easy to use with syringe, pump or a chromatography system.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

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資料

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

すべて表示

プロトコル

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

Characteristics of Glutathione Sepharose® Products

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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