GE17-5282-01

GSTrap^™ High Performance

Cytiva 17-5282-01, pack of 1 × 5 mL

• 別名: GSTrap Column
• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• ligand: glutathione
• 包装: pack of 1 × 5 mL
• メーカー／製品名: Cytiva 17-5282-01
• パラメーター: flow rate; <20 mL/min flow rate; 42 psi (H₂O at room temperature.)
• ベッドサイズ: 16 mm × 25 mm
• ベッド容積: 5 mL
• カラム内径: 16 mm
• マトリックス: highly cross-linked 6% agarose
• 平均部品サイズ: 34 μm
• cleaning: 3-12
• working range: 3-12
• キャパシティ: >7 mg/mL binding capacity (medium)

詳細

詳細

GSTrap^™ HP columns are prepacked with Glutathione Sepharose^™ High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

アプリケーション

GSTrap^™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose^™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged Proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose^™ High Performance. GST-tagged Proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.

特徴および利点

• High resolution purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Fast, one-step purification.
• Easy to use with syringe, pump or a chromatography system.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 1