GE17-5130-02

GSTrap^™ Fast Flow

Cytiva 17-5130-02, pack of 2 × 1 mL

• UNSPSCコード: 23151817
• NACRES: NA.25

特性

• ligand: glutathione and 10-carbon linker arm
• 包装: pack of 2 × 1 mL
• メーカー／製品名: Cytiva 17-5130-02
• パラメーター: <4 mL/min flow rate; 42 psi
• ベッドサイズ: 7 mm × 25 mm
• ベッド容積: 1 mL
• カラム内径: 7 mm
• マトリックス: highly cross-linked 4% agarose
• 平均直径: 90 μm
• cleaning: 3-12
• working range: 3-12
• キャパシティ: >10 mg/mL binding capacity

詳細

詳細

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast FLow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

アプリケーション

GST*-tagged Proteins can be purified directly from pretreated bacterial lysates using GSTrapTM FF.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods. The medium, Glutathione SepharoseTM 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTATM design or FPLCTM System.

特徴および利点

• Fast and simple one-step purification of GST-tagged Proteins.
• Mild elution conditions preserving Protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast FLow provides good fLow properties.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids