おすすめの製品
物質
polypropylene (PP)
分子量
1000-5000 Da (globular proteins)
包装
pkg of 1 ea
メーカー/製品名
Cytiva 17-5087-01
保管条件
dry media
パラメーター
<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)
ベッド容積
53 mL
カラムL × 内径
10 cm × 26 mm
Matrix
cross-linked dextran
polymeric
マトリックス活性基
dextran-epichlorohydrin phase
粒径
17-132 μm (dry)
cleaning
2-13
動作範囲
2-13
分離法
size exclusion (SEC)
関連するカテゴリー
詳細
特徴および利点
- Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
- Replaces dialysis for desalting and therefore saves time and reduces labor.
- Compatible with a laboratory pump or chromatography system such as AKTA design.
- Two or four columns can be used in series for sample sizes of 30 to 60 mL.
適合性
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page describes automated purification of antibodies using ÄKTA chromatography systems.
This page describes automated purification of antibodies using ÄKTA chromatography systems.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.
This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.
プロトコル
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.
This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.
This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.
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