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Merck

GE17-5087-01

Sigma-Aldrich

HiPrep 26/10 Desalting

Cytiva 17-5087-01, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

別名:

Desalting Column, HiPrep Desalting Column

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About This Item

UNSPSCコード:
41115700
NACRES:
SB.52

物質

polypropylene (PP)

分子量

1000-5000 Da (globular proteins)

包装

pkg of 1 ea

メーカー/製品名

Cytiva 17-5087-01

保管条件

dry media

パラメーター

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

ベッド容積

53 mL

カラムL × 内径

10 cm × 26 mm

Matrix

cross-linked dextran
polymeric

マトリックス活性基

dextran-epichlorohydrin phase

粒径

17-132 μm (dry)

cleaning

2-13

動作範囲

2-13

分離法

size exclusion (SEC)

関連するカテゴリー

詳細

HiPrep 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

特徴および利点

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

適合性

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

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法的情報

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

すべて表示

プロトコル

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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