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ligand
quaternary ammonium
詳細
Ion Exchanger Type (value)
包装
pack of 6 mL
メーカー/製品名
Cytiva 17-1179-01
パラメーター
87 psi
ベッドサイズ
16 mm × 30 mm
ベッド容積
6 mL
Matrix
polystyrene/divinylbenzene
平均部品サイズ
15 μm
粒径
15-15 μm
cleaning
1-14
動作範囲
2-12
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
アプリケーション
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
特徴および利点
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows how to perform a purification of His-tagged membrane proteins.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
プロトコル
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
This page discusses how to do a purification with SOURCE™ media from GE Healthcare
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