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Key Documents

GE17-1014-01

Q Sepharose High Performance

Cytiva 17-1014-01, pack of 75 mL

別名:

Q Sepharose Resin

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

ligand

quaternary amine

詳細

Ion Exchanger Type (value)

包装

pack of 75 mL

メーカー/製品名

Cytiva 17-1014-01

マトリックス

6% cross-linked agarose

粒径

24-44 μm

平均部品サイズ

34 μm

cleaning in place

1-14

working range

2-12

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

Q Sepharose High Performance is a strong anion exchange BioProcess resin with high resolution designed for high intermediate and polishing steps in downstream processing.

Q Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. Q Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide.

Q Sepharose High Performance is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess resins range, Q Sepharose High Performance meets industrial demands with security of supply and comprehensive technical and regulatory support.

アプリケーション

Ion Exchange Chromatography (IEX) separates molecules on the bases of differences in their net surface charge. Anion Exchange Chromatography (AIEX) medium interacts with negatively charged proteins and are separated by selective salt elution.

特徴および利点

  • Strong anion exchanger for intermediate and final polishing steps
  • High resolution, high capacity separation with high recovery
  • High chemical stability for effective CIP/sanitization
  • BioProcess resin supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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資料

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

Principles and Standard Conditions for Different Purification Techniques

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プロトコル

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

This page discusses various aspects of sample preparation for chromatographic purification.

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