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Key Documents

GE17-0851-01

Disposable PD 10 Desalting Columns

Cytiva 17-0851-01, pack of 30 pieces

別名:

Disposable desalting columns, PD-10 columns

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

特徴

wetted part: no

包装

pack of 30 pieces

メーカー/製品名

Cytiva 17-0851-01

保管条件

dry media

ベッドサイズ

14.5 mm × 50 mm

ベッド容積

8.3 mL

カラム内径

15 mm

マトリックス

cross-linked dextran

粒径

>50 μm (dry)
85-260 μm

cleaning

2-13

working range

2-13

適合性

suitable for bioprocess medium

関連するカテゴリー

詳細

Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.

The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.

Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol. Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.

特徴および利点

  • Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex® G-25 Medium.
  • Flexible protocols allow runs by gravity flow or centrifugation.
  • Easy to use and equilibrate; apply sample; elute.
  • Preparation of the samples in the range of 1.0 to 2.5 mL by gravity flow and 1.75 to 2.5 mL with centrifugation.
  • Desalt multiple samples in parallel with a desalting capacity >90%.
  • Recovery in the range 70 to >95%.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

法的情報

Sephadex is a registered trademark of Cytiva

よく一緒に購入される製品

ピクトグラム

Exclamation mark

シグナルワード

Warning

危険有害性情報

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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Ye Guan et al.
eLife, 7 (2018-04-06)
Single-cell analysis is pivotal to deciphering complex phenomena like heterogeneity, bistability, and asynchronous oscillations, where a population ensemble cannot represent individual behaviors. Bulk cell-free systems, despite having unique advantages of manipulation and characterization of biochemical networks, lack the essential single-cell

資料

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すべて表示

プロトコル

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

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