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包装
pack of 150 mL
メーカー／製品名
Cytiva 17-0584-10
マトリックス
allyl dextran and N, N’-methylenebisacrylamide
粒径
25-75 μm
平均部品サイズ
50 μm
cleaning in place
2-13
working range
3-11
適合性
suitable for bioprocess medium
詳細
Sephacryl™ High Resolution size exclusion chromatography resins allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by size exclusion chromatography at laboratory and industrial scale.
Sephacryl™ High Resolution chromatography resins is a highly versatile gel filtration resins that offers a wide range of fractionation capabilities. The matrix of Sephacryl™ High Resolution resins is a cross-linked copolymer of allyl dextran and N,N’-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the resins minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl™ HR the resin of choice for routine purification.
Sephacryl™ S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 10³–1 × 10⁵). Sephacryl™ S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³–2.5 × 10⁵). Sephacryl™ S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 10⁴–1.5 × 10⁶). Sephacryl™ S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 10⁴–8 × 10⁶). Sephacryl™ S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 10⁴–2 × 10⁷)
Sephacryl™ High Resolution chromatography resins is a highly versatile gel filtration resins that offers a wide range of fractionation capabilities. The matrix of Sephacryl™ High Resolution resins is a cross-linked copolymer of allyl dextran and N,N’-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the resins minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl™ HR the resin of choice for routine purification.
Sephacryl™ S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 10³–1 × 10⁵). Sephacryl™ S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³–2.5 × 10⁵). Sephacryl™ S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 10⁴–1.5 × 10⁶). Sephacryl™ S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 10⁴–8 × 10⁶). Sephacryl™ S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 10⁴–2 × 10⁷)
特徴および利点
- Cost-efficient gel filtration resins providing high resolution with fast and easy operation.
- Five different fractionation ranges available in Sephacryl™ HR family allows for reproducible separations over a wide molecular weight range.
- Sephacryl™ S-200 High Resolution resin is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 10³ – 2.5 × 10⁵).
- BioProcess™ resin supported for industrial applications and well-established in approved processes.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Sephacryl is a registered trademark of Cytiva
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
プロトコル
This page shows how to perform a separation with a sephacryl column from GE Healthcare which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Column Packing and Preparation for Size Exclusion Chromatography
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