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特徴
autoclavable In wet form (pH 7.0) at 120°C for 30 min
包装
pack of 100 g
メーカー/製品名
Cytiva 17-0010-01
パラメーター
flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
マトリックス
cross-linked dextran
粒径
40-120 μm (dry)
55-165 (wet)
working range
2-13
詳細
Sephadex® G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules > 700 molecular weight. Smallest Exlusion limit in the Sephadex® range.
アプリケーション
Sephadex® is a gel filtration medium prepared by crosslinking Dextran with epichlorohydrin. Different types of Sephadex® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex® G-10 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex® G-50 is available in 4 different particle sizes (Course, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency fractionation with shorter diffusion distances. Cource and Medium are are preferred for large scale group separations where high flow rates and Low operating pressures are required.
特徴および利点
- Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
- Suitable for buffer exchange and cleanup of biological samples, for example peptides, small Proteins, and oligosaccharides larger than 700 molecular weight
- Efficient removal of contaminants such as salts, dyes, and radioactive labels
- Lowest exclusion limit (700 molecular weight) in the Sephadex® G-range
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
Sephadex is a registered trademark of Cytiva
保管分類コード
13 - Non Combustible Solids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
プロトコル
This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.
Column Packing and Preparation for Size Exclusion Chromatography
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