G7900

モノクローナル抗グリコホリンA(α)抗体 マウス宿主抗体

ascites fluid, clone E4

• MDL番号: MFCD00164599
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: E4, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: human
• テクニック: agglutination assay: 1:800 using human erythrocytes; flow cytometry: suitable using bone marrow nucleated cells; indirect immunofluorescence: suitable using bone marrow nucleated cells; western blot: suitable using extracts of human red blood cell ghosts
• アイソタイプ: IgM
• UniProtアクセッション番号: P02724
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GYPA(2993)

詳細

特異性

イムノブロッティングにおいて、抗体はヒト赤血球ゴ-スト抽出物中のα, αδ, α₂のバンドを特異的に認識します。本抗体(クロ-ン番号別名15D4)は、免疫蛍光顕微鏡法やフロ-サイトメトリ-において細胞スメアや組織標本中の骨髄有核細胞の8%に結合します。

免疫原

ヒト胸腺。

アプリケーション

Monoclonal Anti-Glycophorin A (α) antibody produced in mouse is suitable for:

• agglutination assay at a working dilution of 1:800 using human erythrocytes
• flow cytometry using bone marrow nucleated cells
• indirect immunofluorescence using bone marrow nucleated cells
• western blot using extracts of human red blood cell ghosts

生物化学的／生理学的作用

Glycophorins (GP) are sialic acid-rich polypeptides (sialoglycoproteins) that are part of the erythrocyte membrane. They are denoted α, β, γ, δ based on the decreasing molar mass. GPA and GPB are the major constituents of the red cells. They may be present as single polypeptides (α and δ), as stable homodimers (α2 and δ2) and heterodimers (αδ). Depending upon the amino acid residues at positions 1 and 5, GPA carries blood group M or N. GPA is associated exclusively with erythroid cells. It is expressed in pronormoblasts and later erythroid cells. GPA has a cytoplasmic domain that interacts with cytoskeletal structure upon induction by binding to ligand. This interaction improves RBC membrane rigidity and reduces deformability.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable