リコンビナント
expressed in baculovirus infected Sf21 cells
品質水準
アッセイ
>97% (SDS-PAGE)
形状
lyophilized powder
有効性
0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)
分子量
55 kDa (glycosylated form)
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
UniProtアクセッション番号
保管温度
−20°C
遺伝子情報
mouse ... Fas(14102)
詳細
Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.
アプリケーション
Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).
生物化学的／生理学的作用
Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).
物理的形状
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.
アナリシスノート
Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
カルタヘナ法
カルタヘナ法
Jan Code
F8799-50UG-PW:
F8799-BULK:
F8799-50UG:
F8799-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Davidson D Ateh et al.
Biomaterials, 32(33), 8538-8547 (2011-08-10)
The CD95/CD95L receptor-ligand system is mainly recognised in the induction of apoptosis. However, it has also been shown that CD95L is over-expressed in many cancer types where it modulates immune-evasion and together with its receptor CD95 promotes tumour growth. Here
Cheng Jin et al.
Medical science monitor : international medical journal of experimental and clinical research, 21, 630-637 (2015-02-28)
The CD95 gene plays a key role in regulating cell growth and tumor genesis. To date, several publications have focused on the CD95 rs1800682A/G site polymorphism and various types of tumors in Asians; however, this association is still controversial and
B A Treviño-Talavera et al.
Genetics and molecular research : GMR, 13(3), 4831-4838 (2014-07-26)
Primary Sjögren's syndrome (pSS) is a chronic systemic autoimmune disease characterized by lymphocytic infiltration of exocrine glands. Soluble Fas receptor (sFas) has been suggested as a Fas-mediated apoptosis blocker that could impair clonal deletion in infiltrated autoreactive cells. The FAS
J Cheng et al.
Science (New York, N.Y.), 263(5154), 1759-1762 (1994-03-25)
Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because
A Oehm et al.
The Journal of biological chemistry, 267(15), 10709-10715 (1992-05-25)
The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the
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