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由来生物
mouse
品質水準
リコンビナント
expressed in NSO cells
アッセイ
>90% (SDS-PAGE)
フォーム
lyophilized powder
有効性
0.04-2.5 ng/mL
分子量
monomer calculated mol wt 85.3 kDa
100-110 kDa by SDS-PAGE (reducing)
包装
pkg of 200 μg
保管条件
avoid repeated freeze/thaw cycles
テクニック
ligand binding assay: suitable
不純物
endotoxin, tested
UniProtアクセッション番号
保管温度
−20°C
遺伝子情報
mouse ... Ephb2(13844)
詳細
EphB2 (Epherin receptor B2) is a receptor tyrosine kinase (RTK), which belongs to a 13 member family of ephrin receptors. This family is divided into two classes A and B. EphB2 belongs to EphB group, which contains five members. The ligands to this group of receptors are named ephrin-B1-B3. It is a bidirectional functioning membrane protein.
アプリケーション
EphB2/Fc Chimera from mouse has been used for the investigation of the role of ephrinB-EphB signaling in the induction and maintenance of hyperalgesia, hyperexcitability of neurons of DRG (dorsal root ganglion) and hyperexcitability and elevated synaptic plasticity of neurons of DH (dorsal horn) regions of brain.
生物化学的／生理学的作用
EphB2 (Epherin receptor B2) is involved in the development and plasticity of glutamatergic synapses, and their interaction with NMDA receptor. It can also regulate the processing of acute inflammatory pain, and mechanical allodynia as a result of nerve crush. Activation of this receptor results in recruitment and activation of Src-protein kinases. These, in turn, phosphorylate and activate NMDA receptor. It regulates the function of platelets and the development of thrombus. It is also responsible for the retraction of clots, and modulates independent platelet function in a contact-independent manner. EphB2 signaling is also involved in the modulation of axonal sprouting, which is induced by lesions.
Member of the Eph receptor tyrosine kinase family shown to bind ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis, and in arterial/venous demarcation of the developing vasculature.
その他情報
C末端6Xヒスチジンタグ付加ヒトIgG1 Fc領域をポリペプチドリンカ-を介して融合したマウスEphB2(アミノ酸配列1~548)の細胞外ドメイン。
物理的形状
凍結乾燥品(20mM Tris溶液, pH 8.0, 0.2 μmフィルタ-滅菌済み)。
アナリシスノート
生物活性は、ELISAによりリコンビナントマウスエフリン-B2/Fcへの結合能を測定しています。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
E9402-BULK:
E9402-200UG:
E9402-VAR:
E9402-200UG-PW:
Sakthivel Vaiyapuri et al.
Blood, 125(4), 720-730 (2014-11-06)
The Eph kinases, EphA4 and EphB1, and their ligand, ephrinB1, have been previously reported to be present in platelets where they contribute to thrombus stability. Although thrombus formation allows for Eph-ephrin engagement and bidirectional signaling, the importance specifically of Eph
Paul A Nakamura et al.
Neural development, 8, 2-2 (2013-02-06)
Studies of developmental plasticity may provide insight into plasticity during adulthood, when neural circuitry is less responsive to losses or changes in input. In the mammalian auditory brainstem, globular bushy cell axons of the ventral cochlear nucleus (VCN) innervate the
Wen-Tao Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 23(1), 90-98 (2008-09-06)
Cellular and molecular mechanisms underlying opioid tolerance and dependence remain elusive. We investigated roles of EphB receptor tyrosine kinases--which play important roles in synaptic connection and plasticity during development and in the matured nervous system--in development and maintenance of physical
Yuan Han et al.
Molecular pain, 4, 60-60 (2008-11-26)
EphB receptor tyrosine kinases, which play important roles in synaptic connection and plasticity during development and in matured nervous system, have recently been implicated in processing of pain after nerve injury and morphine dependence. Subtypes of the EphB receptors that
Xue-Jun Song et al.
Pain, 139(1), 168-180 (2008-05-02)
Bidirectional signaling between ephrins and Eph receptor tyrosine kinases was first found to play important roles during development, but recently has been implicated in synaptic plasticity and pain processing in the matured nervous system. We show that ephrinB-EphB receptor signaling
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