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タグ
Met-FLAG tagged
グレード
for molecular biology
形状
buffered aqueous solution
ペプチドタグ位置
N-terminal
輸送温度
dry ice
保管温度
−20°C
詳細
pFLAG-CMV™-4 is a 6.2 kb expression vector used for transient or stable expression in mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression of a properly inserted open reading frame as an N-terminal Met-FLAG® fusion protein.
The pFLAG-CMV-4 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E.coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available for Sigma Aldrich.
pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
The pFLAG-CMV-4 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E.coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The FLAG epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available for Sigma Aldrich.
pFLAG-CMV-4-BAP Control Plasmid is a 7.7 kb derivative of pCMV5 used for transient intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
Vector Maps and Sequences
構成
- pFLAG-CMV™-4 Expression Vector 20 μg (E1775) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
- pFLAG-CMV™-4-BAP Control Plasmid 20 μg (C4722) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
原理
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG®-fusion constructs. The aminoglycoside phosphotransferase II gene (neo-r) confers resistance to aminoglycosides such as G 418 allowing for selection of stable transfectants.
法的情報
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC
関連製品
保管分類コード
10 - Combustible liquids
適用法令
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毒物及び劇物取締法
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PRTR
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消防法
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労働安全衛生法名称等を表示すべき危険物及び有害物
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カルタヘナ法
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Jan Code
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試験成績書（COA）
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