E6781

Anti-eIF4ENIF1 (N-terminal) ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Anti-4E-T, Anti-Clast4, Anti-Eukaryotic translation initiation factor 4E nuclear import factor 1, Anti-Eukaryotic translation initiation factor 4E transporter, Anti-eIF4E-T
• MDL番号: MFCD09753854
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 100 kDa
• 交差性: human
• テクニック: immunoprecipitation (IP): 5-10 μL using HeLa cell lysates; indirect immunofluorescence: 5-10 μg/mL using paraformaldehyde-fixed HEK-293T cells; western blot: 1-2 μg/mL using HeLa cell lysates
• UniProtアクセッション番号: Q9NRA8
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EIF4ENIF1(56478)

詳細

詳細

Eukaryotic translation initiation factor 4E nuclear import factor 1 (eIF4ENIF1) comprises two leucine-rich nuclear export signals and a bipartite nuclear localization signal. eIF4ENIF1 gene is mapped to human chromosome 22q12.2 and is expressed in the human ovary.

特異性

Anti-eIF4ENIF1(N-terminal) specifically recognizes human eIF4ENIF1.

免疫原

synthetic peptide corresponding amino acids 1-19 of human eIF4ENIF1, conjugated to KLH via a C-terminal added cysteine residue. This sequence is specific to human.

アプリケーション

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit is suitable for the following applications:

• Immunoprecipitation at a concentration of 5-10μL using HeLa cell lysates
• Indirect immunofluorescence at a concentration of 5-10μg/mL using paraformaldehyde-fixed HEK-293T cells
• Western blotting at a concentration of 1-2μg/mL using HeLa cell lysates

Anti-eIF4ENIF1 (N-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

生物化学的／生理学的作用

Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein encoded by the EIF4ENIF1 gene in humans. It is a nucleocytoplasmic shuttling protein that mediates the import of eIF4E into the nucleus. 4E-transporter (4E-T) is one of several proteins that bind the mRNA 5′cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. Its interaction with eIF4E is a priming event in inducing messenger ribonucleoprotein rearrangement and transition from translation to decay. Eukaryotic initiation factor 4E plays an important role in controlling cell growth. 4E-T is essential for the assembly of processing bodies and its overexpression triggers the movement of eIF4E into the processing bodies.

eIF4ENIF1 (also known as 4E-T or 4E-transporter) colocalizes with other mRNA binding/processing proteins to processing (P)-bodies. Its interaction with eIF4E is essential for targeting of mRNAs to P-bodies. eIF4ENIF1 competes with eIF4G for binding to eIF4E and preventing the formation of the eIF4F complex thus inhibits translation. Together with 4E-BPs, it participates in cell response to prolonged hypoxia, sequestering eIF4E, and affecting gene expression. A nonsense mutation in the eIF4ENIF1 gene is implicated in the Primary Ovarian Insufficiency.

物理的形状

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)