E5906

抗eIF4E ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-CBP, Anti-Eukaryotic intiation factor 4E, Anti-MGC111573, Anti-eIF4E1, Anti-eIF4EL1, Anti-eIF4F
• MDL番号: MFCD01633313
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 25 kDa
• 化学種の反応性: rat (predicted), human, mouse
• 濃度: ~1 mg/mL
• テクニック: immunocytochemistry: 5-10 μg/mL using paraformaldehyde-fixed 3T3 cells; immunoprecipitation (IP): 2-5 μL using HeLa cell lysates; western blot: 0.5-1 μg/mL using HeLa cell lysates
• UniProtアクセッション番号: P06730
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EIF4E(1977) ; mouse ... Eif4e(13684) ; rat ... Eif4e(117045)

詳細

詳細

The translational factor eukaryotic translation initiation factor 4E (eIF4E), also known as mRNA cap-binding protein(CBP) or EIF4E1 is a central component of translation initiation and regulation in eukaryotic cells. eIF4E exists as both a free form and as part of a multiprotein complex termed eIF4F. Although mainly expressed in the cytoplasm, a fraction of eIF4E localizes to the nucleus, and some of the biological effects of eIF4E might be effected by a nuclear function.

免疫原

synthetic peptide corresponding to amino acids 21-37 of human eIF4E, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse.

アプリケーション

Anti-eIF4E antibody produced in rabbit has been used in immunoblotting

生物化学的／生理学的作用

The translational factor eukaryotic translation initiation factor 4EeIF4E regulates the recruitment of mRNAs to the ribosome. Its interaction with mRNA cap structure enhances its binding to eIF4G and 40S ribosomal subunit. eIF4Eplays a role in diverse biological processes including embryonic development, cell cycle progression, synaptic plasticity, and cancer. eIF4E is subjected to tight regulation both at the level of its expression and by control of its activity by phosphorylation mitogen-activated protein kinase (MAPK) interacting protein kinases (by the Mnk kinase). Human P bodies contain the cap-binding protein eIF4E and the related factor eIF4E-transporter (eIF4E-T; eIF4ENIF1), suggesting novel roles for these proteins in targeting mRNAs for 5′ to 3′ degradation.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)