E4531

Anti-ELKS antibody, Mouse monoclonal

clone ELKS-30, purified from hybridoma cell culture

• 別名: Anti-KIAA1081
• MDL番号: MFCD08705584
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ARR; ICC; IP; WB
• クローン: ELKS-30, monoclonal
• 化学種の反応性: rat, chicken, bovine, human, mouse
• citations: 12
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using HeLa cell extract

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: ELKS-30, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~120 kDa
• 化学種の反応性: rat, chicken, bovine, human, mouse
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.5-1 μg/mL using HeLa cell extract
• アイソタイプ: IgG2a
• UniProtアクセッション番号: Q8IUD2
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ERC1(23085) ; mouse ... Erc1(111173)

詳細

詳細

ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap.

Monoclonal Anti-ELKS (mouse IgG2a isotype) is derived from the hybridoma ELKS-30 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human ELKS. ELKS, also known as KIAA1081, is an essential regulatory subunit of the IKK complex. It was named ELKS since 44.1% of the sequence is composed of glutamic acid (E), leucine (L), lysine (K) and serine (S) residues. The protein contains 948 amino acids with nine -helical coiled-coil domains including periodic heptad repeats that predict dimer formation. Its highest expression is in heart, placenta, lung, brain, thyroid and testis.

アプリケーション

Monoclonal Anti-ELKS antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• fluorescence immunohistochemistry
• immunocytochemistry
• immunoblotting

生物化学的／生理学的作用

ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap. ERC1 encodes a regulator of neurotransmitter release and acts as the best gene candidate contributing to this phenotype as well as to the autism spectrum disorder ASD. ERC1a (an isoform of ERC1) is associated with other proteins for effective migration and tumor cell invasion by stabilizing the activity at the cell front. Depletion of any of the associated protein negatively affects invasion, migration on extracellular matrix, lamellipodial persistence and the internalization of active integrin β1 receptors needed for adhesion turnover at the front of the cell.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)