E2520

モノクローナル抗上皮増殖因子抗体 マウス宿主抗体

clone EGF-10, purified from hybridoma cell culture

• 別名: Anti-CEGF
• MDL番号: MFCD00239669
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: EGF-10, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 6 kDa
• 化学種の反応性: human
• テクニック: dot blot: 0.1 μg/mL using recombinant human EGF; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue; indirect ELISA: suitable; microarray: suitable; neutralization: suitable; radioimmunoassay: suitable; western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P01133
• アプリケーション: research pathology
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EGF(1950)

詳細

詳細

Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets.

特異性

天然およびリコンビナントヒトEGFと特異的に反応します。抗体は、マウスEGFに対する交差反応性はありません。

免疫原

リコンビナントヒトEGF。

アプリケーション

Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining.

Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.

生物化学的／生理学的作用

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.

Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization.

物理的形状

0.01M PBS溶液 (pH 7.4)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves