E2279

Monoclonal Anti-Endonuclease VIII antibody produced in mouse

~1.5 mg/mL, clone E8-122, purified immunoglobulin, buffered aqueous solution

• 別名: Monoclonal Anti-Endo VIII
• MDL番号: MFCD03792417
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: E8-122, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~30 kDa by SDS-PAGE
• 化学種の反応性: E. coli
• 濃度: ~1.5 mg/mL
• テクニック: indirect ELISA: suitable; western blot: 10-20 μg/mL using recombinant endonuclease VIII (Cat No. E0651)
• アイソタイプ: IgM
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Monoclonal Anti- Endonuclease VIII (mouse IgM isotype) is derived from the E8-122 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice . Endonuclease VIII (Endo VIII) from E. coli is a product of the nei gene. It is a DNA N-glycosylase and abasic site lyase (AP1 lyase. It comprises C-terminal Cys4-type β/β-antiparallel zinc finger with an arginine residue (Arg-252).

特異性

Monoclonal Anti-Endonuclease VIII recognizes endonuclease VIII from E. coli (approx. 30 kDa).

免疫原

recombinant full length endonuclease VIII from Escherichia coli.

アプリケーション

Monoclonal Anti-Endonuclease VIII antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

生物化学的／生理学的作用

Endonuclease VIII (Endo VIII) from E. coli is a DNA repair protein that recognizes and removes modified pyrimidines, such as thymine glycol (Tg) and 5,6,dihydrothymine (DHT), from DNA.. Its modified base substrate specificity overlaps the endonuclease III (endo III, nth) substrate specificity. This cross-reactivity is manifested in E. coli mutants. E. coli nth and nei mutants are either not sensitive or are slightly more sensitive to ionizing radiation and hydrogen peroxides than the wild type. The nei-nth double mutant is hypersensitive to oxidative stress. The mechanism used by Endo VIII to cleave the DNA backbone (lyase activity) is a β-δ elimination, resembling the action of formamidopyrimidine-DNA glycosylase (fpg) protein.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable