E0885

エンテロキナーゼ ブタ腸由来

lyophilized powder, ≥100 units/mg protein

• 別名: エンテロペプチダーゼ
• CAS番号: 9014-74-8
• Enzyme Commission number: 3.4.21.9 (BRENDA, IUBMB)
• EC Number: 232-761-1
• MDL番号: MFCD00131020
• UNSPSCコード: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

特性

• 形状: lyophilized powder
• 品質水準: 200
• 比活性: ≥100 units/mg protein
• 分子量: 150 kDa
• 精製方法: chromatography
• 組成: Protein, ≥20% Lowry
• その他の活性: aminopeptidase ≤1.5%; trypsin ≤1%, free
• 保管温度: −20°C

詳細

アプリケーション

Enterokinase from porcine intestine has been used in a study to investigate complementary DNA cloning and sequencing of rat enteropeptidase. Enterokinase from porcine intestine has also been used to learn more about the insulinotropic region of the gastric inhibitory polypeptide.

The enzyme from Sigma has been used to activate zymogens in order to detect trypsin activity. The study to investigated the structural and evolutionary consequences of unpaired cysteines in trypsinogen. The product has been used to measure trypsin while studying the effect of pesticide induced alterations in gene expression in the lobster, Homarus americanus The enzyme from Sigma has been used to develop a novel assay for measuring levels of lipid-free apoA-I in the presence of lipid-bound apoA-I. Enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form resulting in an N-terminal fragment of 22 kDa. It has also been used in a study to examine the effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin.

生物化学的／生理学的作用

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)₄-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

単位の定義

1ユニットは、pH 5.6、25°C、1分間で、1.0 nmolのトリプシンをトリプシノーゲンから生成する酵素量です。

物理的形状

凍結乾燥品(リン酸ナトリウムバッファ-塩含有)

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)