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特異性
Unique Restriction Sites (Methylated DNA) are: Apa I,Nae I, Nar I, Nhe I, PaeR7 I, SnaB I, Xba I and Xho I.
アプリケーション
Lambda Phage DNA, Non-methylated from Escherichia coli host strain GM119 is suitable for use as a substrate for restriction enzymes. It was used as substrate for Prevotella ruminicola DNase activity studies. It was also used as a GC content standard in genome sequencing of 16S rDNA sequences of Cellvibrio japonicas.
Lambda Phage DNA, Non-methylated from Escherichia coli host strain GM119 (rm-,dam-,dcm-) has been used:
- to calibrate spectrophotometer (LKB) and HPLC (high performance liquid chromatography) method for determination of bacterial genomic DNA G+C content
- to amplify the λ; exonuclease gene by polymerase chain reaction (PCR)
- in DNase activities
The lambda phage has an icosahedral head and a long tail terminating in a single fiber. At both ends of the 5′ termini are complementary 12-nucleotide single strand sequences that contribute to the cohesive ends (cos region) of the DNA. The tail of the phage latches on the host outer membrane receptor and injects phage DNA into the cell. The phage converts the E. coli to a lysogenic state in which the phage functions are repressed and the phage genome may remain dormant (prophage) for a long time. This property is seen in bacteriophages that carry CII and CIII genes that are responsible for CI expression. Bacteriophages with CI mutation in the CI gene are able to maintain a lysogenic state at defined temperatures.
Infecting E. coli strain GM 119 with lambda C1857 strain creates E. coli lysogen cultures. The phage is released from E. coli cell pellets by lysing with a high salt buffer, pH 8.0. The crude mixture is passed through a series of enzymatic steps, multiple cesium gradients, and phage DNA is dialyzed against 1 mM Tris-HCl, pH 8.0, and 1 mM magnesium chloride. The DNA is finally extracted by phenol-chloroform solution.
Infecting E. coli strain GM 119 with lambda C1857 strain creates E. coli lysogen cultures. The phage is released from E. coli cell pellets by lysing with a high salt buffer, pH 8.0. The crude mixture is passed through a series of enzymatic steps, multiple cesium gradients, and phage DNA is dialyzed against 1 mM Tris-HCl, pH 8.0, and 1 mM magnesium chloride. The DNA is finally extracted by phenol-chloroform solution.
物理的形状
Phage DNA is isolated from infected E. coli, passed through a series of enzymatic steps before final phenol-chloroform extraction. This non-methylated lambda DNA is completely digested by Bcl I, Cla I, Mbo I, Mbo II, Taq I or Xba I whereas methylated lambda DNA(product number D3779) is only partially cleaved. The unique restriction sites are Apa I,Nae I, Nar I, Nhe I, PaeR7 I, SnaB I, Xba I and Xho I.
基質
Non-methylated lambda DNA is completely digested by Bcl I, Cla I, Mbo I, Mbo II, Taq I or Xba I whereas methylated lambda DNA is only partially cleaved.
保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D3654-1UN:
D3654-5UN:
D3654PROC:
D3654-10UN:
D3654-BULK:
D3654-VAR:
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Janine G Borgaro et al.
Nucleic acids research, 41(7), 4198-4206 (2013-03-14)
In T4 bacteriophage, 5-hydroxymethylcytosine (5hmC) is incorporated into DNA during replication. In response, bacteria may have developed modification-dependent type IV restriction enzymes to defend the cell from T4-like infection. PvuRts1I was the first identified restriction enzyme to exhibit specificity toward
Distribution of restriction endonucleases among some entomopathogenic strains of Bacillus sphaericus
Zahner V and Priest FG
Letters in Applied Microbiology, 24, 483-487 (1997)
Reclassification of `Pseudomonas fluorescens subsp. cellulosa?NCIMB 10462 (Ueda et al. 1952) as Cellvibrio japonicus sp. nov. and revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio fulvus sp. nov., nom. rev.
Humphry D R, et al.
International Journal of Systematic and Evolutionary Microbiology, 53(2), 393-400 (2003)
Allosteric ring assembly and chemo-mechanical melting by the interaction between 5?-phosphate and lambda exonuclease
Yoo J and Lee G
Nucleic Acids Research, 43(22), 10861-10869 (2015)
David R Humphry et al.
International journal of systematic and evolutionary microbiology, 53(Pt 2), 393-400 (2003-04-25)
'Pseudomonas fluorescens subsp. cellulosa' NCIMB 10462 has been demonstrated by a polyphasic taxonomic approach to be a member of the genus Cellvibrio. 16S rDNA sequence analysis suggests that this is the only genus that could accept this specimen. The sequence
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