D219

Monoclonal Anti-Dihydropyridine Receptor (α₂ Subunit) antibody produced in mouse

clone 20A, ascites fluid, buffered aqueous solution

• 別名: Anti-CACNA2, Anti-CACNL2A, Anti-CCHL2A, Anti-LINC01112, Anti-lncRNA-N3
• MDL番号: MFCD01323189
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 20A, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 143 kDa (reduced); antigen 220 kDa (non-reduced)
• 化学種の反応性: rat, mouse, rabbit, guinea pig
• テクニック: immunohistochemistry (frozen sections): 1:500; western blot: 1:500
• アイソタイプ: IgG2a
• UniProtアクセッション番号: O08532
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Cacna2d1(12293) ; rat ... Cacna2d1(25399)

詳細

詳細

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

特異性

DHPレセプタ-のα₂サブユニットと特異的に反応します。ラット、マウス、モルモット、ウサギの骨格筋や心筋のほか、ラット脳や肺組織においてα₂ サブユニットとの強い反応性が観察されます。ラット腎臓、肝臓、脾臓組織ではこれより弱い反応性が認められます。免疫組織化学において、Z線の反対側に三つ組(triad)構造を表す点状に分散した染色による二重の列がみられます。

免疫原

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:

• immunofluorescent histochemistry
• surface plasmon resonance binding
• immunohistochemistry (IHC)
• immunoblotting

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

生物化学的／生理学的作用

Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.

物理的形状

PBS溶液(0.05%アジ化ナトリウム含有)。

その他情報

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)