D0180

モノクローナル抗DOPAデカルボキシラーゼ(DDC)抗体 マウス宿主抗体

clone DDC-109, ascites fluid

• MDL番号: MFCD00162261
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: DDC-109, monoclonal
• 分子量: antigen ~50 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: rabbit, sheep, guinea pig, monkey, rat, human, bovine, canine
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1:1,000 using bovine brain extract
• アイソタイプ: IgG2b
• UniProtアクセッション番号: P20711
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... DDC(1644) ; rat ... Ddc(24311)

詳細

詳細

DDC is a 85-90 kDa molecular weight protein that is mainly expressed in brain, kidney and liver.

Monoclonal Anti-DOPA Decarboxylase (DDC) (mouse IgG2b isotype) is derived from the DDC-109 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

アプリケーション

Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse may also be used in immunoprecipitation, immunocytochemistry and ELISA applications. In immunocytochemical labeling of brain tissue, the product stains the monoaminergic cells and proximal dendrites, but not cortical fibers. It is suitable for use in western blotting at a working dilution of 1:1000 using bovine brain extract. It was used for immunoblotting and immunoprecipitation of AADC (aromatic amino acid decarboxylase) from rat striatum in a study. It was used as a primary antibody for western blotting and co-immunoprecipitation studies using human embryonic kidney cells and human neuroblastoma cells.

生物化学的／生理学的作用

DOPA Decarboxylase (DDC), which requires pyridoxal phosphate for its activity, decarboxylates 5-hydroxytryptophan to form serotonin. It also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic neurons.

DOPA decarboxylase (DDC, EC 4.1.1.28), catalyzes the decarboxylation of the 5-hydroxytrptophan (5-HTP) to 5-HT. It requires pyridoxal phosphate for its activity. This is the enzyme that also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic.. Antibodies that react specifically with the enzyme enable the identification of both noradrenergic, dopaminergic, and serotoninergic systems, since DDC is present and active in all three types of neurons.

免責事項

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