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製品種目
ColorWheel®
品質水準
フォーム
lyophilized
精製方法
ion-exchange chromatography
包装
antibody small pack of 25 μL
環境により配慮した代替製品の特徴
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
テクニック
flow cytometry: suitable
蛍光検出
λex 588 nm; λem 604 nm
適合性
for use with ColorWheel® Antibody (Required, Sold Separately)
環境により配慮した代替製品カテゴリ
, Aligned
輸送温度
ambient
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
詳細
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
iFluor 594 is optimized for labeling antibodies and proteins. It is a pH-independent dye that works over a wide range of pH (3 to 11). It displays an excitation max at 588 nm and an emission max at 604 nm. Its spectral properties are similar to Texas Red® and ALEXA FLUOR™ 594. However, compared to Texas Red®, it is easier to conjugate with R-Phycoerythrin (RPE) with higher conjugation yield and the resulting RPE-iFluor 594 tandem dye offers better FRET efficiency. It exhibits decent stability shows good reactivity and selectivity with amino groups.
iFluor 594 is optimized for labeling antibodies and proteins. It is a pH-independent dye that works over a wide range of pH (3 to 11). It displays an excitation max at 588 nm and an emission max at 604 nm. Its spectral properties are similar to Texas Red® and ALEXA FLUOR™ 594. However, compared to Texas Red®, it is easier to conjugate with R-Phycoerythrin (RPE) with higher conjugation yield and the resulting RPE-iFluor 594 tandem dye offers better FRET efficiency. It exhibits decent stability shows good reactivity and selectivity with amino groups.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
特異性
ColorWheel Antibody-Ready iFluor 594 Dye is designed for use with ColorWheel Dye-Ready antibodies in Flow cytometry.
アプリケーション
Flow Cytometry: Excitation: 588 nm, Emission: 604 nm.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
適合性
Requires ColorWheel® Antibody (Sold Separately)
物理的形状
CWDS-IF594-25UL Set Components
CWDS-IF594-25UL - ColorWheel® Antibody-Ready iFluor 594 Dye Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - ColorWheel® Finishing Buffer Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
CWDS-IF594-25UL - ColorWheel® Antibody-Ready iFluor 594 Dye Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - ColorWheel® Finishing Buffer Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
再構成
Reconstitute each vial by adding 25 μL PBS.
保管および安定性
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.
法的情報
ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
11 - Combustible Solids
WGK
WGK 3
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