ATDC5

99072806, mouse unknown/unspecified, Epithelial-like

• 別名: ATDC-5 Cells
• UNSPSCコード: 41106514

特性

• product name: ATDC5, 99072806
• 由来生物: mouse unknown/unspecified
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse 129 teratocarcinoma AT805 derived

細胞株の説明

The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.

アプリケーション

ATDC5 has been used to study cartilage differentiation.

培地

DMEM: Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO₂; 37°C subculture every 2-3 days.

その他情報

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