8C9

99072805, Reactivity: Anti-CD36, human

• UNSPSCコード: 41106514

特性

• 由来生物: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen,female
• 骨髄腫: NS-1
• 反応性: Anti-CD36, human
• テクニック: cell culture | mammalian: suitable
• アイソタイプ: IgM
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse x Mouse hybridoma

細胞株の説明

The hybridoma line 8C9 was produced by immunizing a female BALB/c mouse i.p. with tissue from a human malignant fibrous histiocytoma once a week for 6 weeks. Mouse spleen cells were fused with myeloma NS-1 cells using PEG and HAT for selection . The 8C9 monoclonal antibody detects a unique epitope on the leukocyte differentiation antigen CD36 (platelet glycoprotein IV or III6) expressed by normal and neoplastic endothelial cells. Immunoreactivity with 8C9 was detected on capillary endothelial cells, adipocytes, monocytes, platelets and the human monocytoid cell line U-937 (Sigma Catalogue number. 85011440). The antibody did not react with erythroid cells, T cells, B cells or HUVECs (human umbilical vein endothelial cells). Immunoblot analysis showed that 8C9 bound to a 97-kDa membrane protein expressed by U-937 cells treated with phorbol ester. Comparative immunohistochemical studies using 8C9 and the monoclonal antibody OKM demonstrated epitopic heterogeneity of the CD36 antigen by normal and neoplastic endothelial cells.

免疫原

Human malignant fibrous histiocytoma

アプリケーション

Study of endothelial cells, CD36 expression analysis

培地

RPMI 1640 + 2mM glutamine + 10% FBS

継代と培養方法

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

その他情報

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