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Key Documents

SHP-77

98110201, human lung, Not specified

別名:

Human SHP-77, SHP-77 Cell Line

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About This Item

UNSPSCコード:
41106514

product name

SHP-77, 98110201

由来生物

human lung

成長モード

Adherent/aggregates in suspension

核型

Not specified

形態

Not specified

製品

Not specified

受容体

Not specified

テクニック

cell culture | mammalian: suitable

関連疾患

cancer

輸送温度

dry ice

保管温度

−196°C

細胞株の由来

Human Caucasian lung small cell carcinoma, large cell variant

細胞株の説明

SHP-77 was established in 1977 being derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung. The patient was a 54 year old male with blood type O, Rh+. The cell line has a variety of unusual characteristics including the morphology of an undifferentiated large cell variant of small cell lung carcinoma (SCLC) but biochemical properties of a classical SCLC. SHP-77 express neural cell adhesion molecule (NCAM), NHK-1, CD56 and the cell surface leucocyte antigen Leu-7(HNK-1;CD57). Other neuroendocrine markers are L-dopa decarboxylase and dense core secretory granules. Gland formation and intracytoplasmic lamellar bodies have been shown by electron microscopy. This cell line is useful as in vitro target in 51Cr and III In release cytotoxicity assays. They are suitable for in vitro nude mice assays for evaluation of immune reactivity of cells and serum from lung cancer patients. In addition, they serve to evaluate the immune status of patients with SCLC who received radiation or chemotherapy treatment. SHP-77 cells are tumorigenic in nude mice and grow as circumscribed nodules without metastases formation. They have a reported doubling time of 96 hours.

アプリケーション

SCLC studies, cytotoxicity assays, immunological studies

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8
D16S539: 11
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 9,11
vWA: 16

培地

RPMI 1640 + 2mM Glutamine + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS).

継代と培養方法

Maintain cultures between 4-9 x 100,000 cells / ml; 5% CO2; 37°C. Cells grow as mixture of loosely adherent and floating clusters.

その他情報

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