BHL-89

98070109, human lymph (axillary), Lymphoblastoid

• UNSPSCコード: 41106514

特性

• product name: BHL-89, 98070109
• 由来生物: human lymph (axillary)
• 成長モード: Suspension
• 核型: Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
• 形態: Lymphoblastoid
• 製品: Not specified
• 受容体: sIgM,CD22,BB1,HLA-ABC,GnRH
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human B-type EBNA negative lymphoma

細胞株の説明

The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

アプリケーション

B lymphoma studies, cancer treatments

培地

RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

継代と培養方法

Maintain cultures between 1-5 x 100,000 cells/ml;5% CO₂; 37°C

その他情報

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