HKT-1097

98061003, hamster kidney, Fibroblast-like

• UNSPSCコード: 41106514

特性

• 製品名: HKT-1097, 98061003
• 由来生物: hamster kidney
• 成長モード: Adherent
• 核型: Modal no. 44
• 形態: Fibroblast-like
• 製品: Not specified
• 受容体: EGF
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Syrian Hamster kidney

細胞株の説明

The cell line HKT-1097 has been established from a 14 month old male Syrian Hamster (Mesocricetus auratus) kidney tumour which has been induced by diethylstilbestrol exposure. HKT-1097 cells express epidermal growth factor (EGF) receptor, Erb-B2 and Erb-B3 as well as vimentin, desmin, glial fibrillary acidic protein (GFAP), TGF alpha, PGP 9.5 and S100 protein and neuron-specific enolase. Karyotype analysis revealed a modal number of 44 chromosomes (41% of cells) and polyploidy in approximately 2% of cells. The cells have a doubling time of about 36 hours.

DNAプロファイル

特記事項なし

培地

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4, i.e., seeding at 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Form a multipolar, loosely packed monolayer.

その他情報

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