MFM-223

98050130, human breast, Epithelial-like

• 別名: MFM223 Cells
• UNSPSCコード: 41106514

特性

• product name: MFM-223, 98050130
• 由来生物: human breast
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Androgen receptor
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human caucasian mammary carcinoma

細胞株の説明

The mammary carcinoma cell line MFM-223 was obtained from the pleural effusion of a postmenopausal breast cancer patient. The patient had not received any prior treatment and the tumour was classified as a widespread ductal mammary carcinoma with the histological grade of III. The cell line is characterised by high androgen, (approximately 100,000 binding sites per cell) and low oestrogen and progesterone receptor levels. Proliferation of cells was inhibited by addition of the androgen dihydrotestosterone at concentrations above 0.01 nM. MFM-223 have a characteristic pattern of isoenzyme polymorphism and of karyotype alterations revealing marker chromosomes and homogenously staining regions. Most cells have been near diploid with 10% displaying near tetraploidy. The cells have been tested to be free of Hepatitis B and C viruses and HIV. MFM-223 is tumourigenic in nude mice.

アプリケーション

Androgen receptor studies, study of mammary carcinoma

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 17

培地

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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