A375-C5 Cell Line human

97011320, human skin, Epithelial-like

• UNSPSCコード: 41106514

特性

• 製品名: A375-C5 Cell Line human, 97011320
• 由来生物: human skin
• 詳細: Human malignant melanoma IL-1 insensitive
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human malignant melanoma IL-1 insensitive

細胞株の説明

The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.

アプリケーション

IL-1 resistance studies, receptor studies

培地

RPMI 1640 + 2mM Glutamine + 10% FBS

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

その他情報

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