OE33 Cell Line human

96070808, human esophagus, Epithelial

• 別名: JROECL33, JROECL33 Cells, OE-33 Cells
• UNSPSCコード: 41106514

特性

• 製品名: OE33 Cell Line human, 96070808, Caucasian esophageal carcinoma
• 由来生物: human esophagus
• 成長モード: Adherent
• 核型: Aneuploid
• 形態: Epithelial
• 製品: Not specified
• 受容体: MHC class I, ICAM-1
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human Caucasian oesophageal carcinoma

細胞株の説明

The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.

アプリケーション

OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.

Study of oesophageal cancer

培地

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.