MOR/0.2R

96042335, human lung, Not specified

• UNSPSCコード: 41106514

特性

• product name: MOR/0.2R, 96042335
• 由来生物: human lung
• 成長モード: Semi-adherent aggregates
• 核型: Not specified
• 形態: Not specified
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human lung adenocarcinoma, drug-resistant

細胞株の説明

The drug resistant cell line MOR/0.2R has been derived from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). MOR/0.2R accumulate lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance associated protein (MRP). Expression of a 190kDa membrane protein associated with the degree of drug-resistance has been indicated. Cells grow as easily detaching aggregates.

アプリケーション

Analysis of acquired drug resistance, MRP studies

培地

RPMI 1640 + 2mM Glutamine + 0.2μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

その他情報

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