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製品名
MOR/CPR, 96042333
由来生物
human lung
成長モード
Aggregates in suspension
核型
Not specified
形態
Not specified
製品
Not specified
受容体
Not specified
テクニック
cell culture | mammalian: suitable
関連疾患
cancer
輸送温度
dry ice
保管温度
−196°C
細胞株の由来
Human lung adenocarcinoma, drug-resistant
細胞株の説明
MOR/CPR has been developed by growing the parent line, MOR, in increasing concentrations of cisplatin. The cells are cross-resistant to melphalan but show little or no cross-resistance to other platinum compounds. Cisplatin accumulation has been found to be reduced compared to the parent line.
アプリケーション
Analysis of acquired drug resistance
培地
RPMI 1640 + 2mM Glutamine + 1μg/ml cisplatin + 10% Foetal Bovine Serum (FBS).
継代と培養方法
Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a
その他情報
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