NCI-H69VCR/R

96042330, human lung, Not specified

• UNSPSCコード: 41106514
• eCl@ss: 32190102

特性

• 製品名: NCI-H69VCR/R, 96042330
• 由来生物: human lung
• 成長モード: Aggregates in suspension
• 核型: Not specified
• 形態: Not specified
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Human small cell lung cancer, drug-resistant

細胞株の説明

NCI-H69 VCR/R has been derived from the parent small cell lung cancer line NCI-H69 (Sigma Catalogue number. 91091802) It is recommended to culture cells without drug upon resuscitation; add vincristine once growth is fully established. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

アプリケーション

Drug-resistance studies

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

培地

RPMI 1640 + 2mM Glutamine + 2.0 μg/ml vincristine + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Maintain cultures between 3-9x 100,000 cells/ml; 5% CO₂; 37ºC. Resuscitate in drug-free medium, add after growth has been established.

その他情報

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