M15

95102517, mouse kidney, Epithelial

• UNSPSCコード: 41106514

特性

• 製品名: M15, 95102517
• 由来生物: mouse kidney
• 成長モード: Adherent
• 核型: Hyperdiploid
• 形態: Epithelial
• 製品: WT1 (expressed gene)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse mesonephric epithelium, polyoma virus large T transformed

細胞株の説明

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

アプリケーション

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

培地

DMEM + 2mM グルタミン + 10％ ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

その他情報

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